A Rapid PCR‐RFLP Method for Monitoring Genetic Variation among Commercial Mushroom Species*
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Wiley
Abstract
Description
We report the development of a simplified procedure for restriction fragment length polymorphism (RFLP)
analysis of mushrooms. We have adapted standard molecular techniques to be amenable to an undergraduate
laboratory setting in order to allow students to explore basic questions about fungal diversity and
relatedness among mushroom species. The streamlined protocols allowed students to practice important
molecular techniques within the context of self-designed investigative projects. This laboratory experience
provided opportunities for students to practice strategies for examining molecular diversity among species.
Keywords
RFLP analysis, Biotechnology education, Phylogenetic analysis, Phylogenetic analysis, Mushrooms