Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis

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dc.creatorLemmer, Yolandy
dc.creatorKalombo, Lonji
dc.creatorPietersen, Ray-Dean
dc.creatorJones, Arwyn T.
dc.creatorSemete-Makokotlela, Boitumelo
dc.creatorVan Wyngaardt, Sandra
dc.creatorRamalapa, Bathabile
dc.creatorStoltz, Anton C.
dc.creatorBaker, Bienyameen
dc.creatorVerschoor, Jan A.
dc.creatorSwai, Hulda
dc.creatorde Chastellier, Chantal
dc.date2019-10-18T06:22:47Z
dc.date2019-10-18T06:22:47Z
dc.date2015-06-06
dc.date.accessioned2022-10-25T09:21:04Z
dc.date.available2022-10-25T09:21:04Z
dc.descriptionResearch Article published by Elsevier
dc.descriptionThe appearance of drug-resistant strains of Mycobacterium tuberculosis (Mtb) poses a great challenge to the development of novel treatment programmes to combat tuberculosis. Since innovative nanotechnologiesmight alleviate the limitations of current therapies, we have designed a new nanoformulation for use as an anti-TB drug delivery system. It consists of incorporating mycobacterial cellwallmycolic acids (MA) as targeting ligands into a drug-encapsulating Poly DL-lactic-co-glycolic acid polymer (PLGA), via a double emulsion solvent evaporation technique. Bonemarrow-derivedmousemacrophages, either uninfected or infectedwith differentmycobacterial strains (Mycobacterium avium, Mycobacterium bovis BCG or Mtb), were exposed to encapsulated isoniazid-PLGA nanoparticles (NPs) using MA as a targeting ligand. The fate of the NPs was monitored by electron microscopy. Our study showed that i) the inclusion of MA in the nanoformulations resulted in their expression on the outer surface and a significant increase in phagocytic uptake of the NPs; ii) nanoparticle-containing phagosomes were rapidly processed into phagolysosomes, whether MA had been included or not; and iii) nanoparticlecontaining phagolysosomes did not fuse with non-matured mycobacterium-containing phagosomes, but fusion events with mycobacterium-containing phagolysosomes were clearly observed.
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dc.identifierhttp://dx.doi.org/10.1016/j.jconrel.2015.06.005
dc.identifierhttp://dspace.nm-aist.ac.tz/handle/123456789/501
dc.identifier.urihttp://hdl.handle.net/123456789/95418
dc.languageen
dc.publisherElsevier
dc.subjectTuberculosis
dc.subjectMycolic acids
dc.subjectNanodrug delivery
dc.subjectElectron microscopy
dc.titleMycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis
dc.typeArticle

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