Molecular Characterization and Immune Response to Lipopolysaccharide (LPS) of the Suppressor of Cytokine Signaling (SOCS)-1, 2 And 3 Genes in Nile Tilapia (Oreochromis Niloticus)

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dc.creatorLiu, Caizhi Z.
dc.creatorHe, An Y.
dc.creatorChen, Li-Qiao
dc.creatorSamwel Mchele Limbu
dc.creatorWang, Ya W
dc.creatorZhang, Mei L.
dc.creatorDu, Zhen-Yu
dc.date2016-06-26T17:11:03Z
dc.date2016-06-26T17:11:03Z
dc.date2016
dc.descriptionSuppressor of cytokine signaling (SOCS) proteins are inverse feedback regulators of cytokine and hormone signaling mediated by the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway that are involved in immunity, growth and development of organisms. In the present study, three SOCS genes, SOCS-1, SOCS-2 and SOCS-3, were identified in an economically important fish, Nile tilapia (Oreochromis niloticus) referred to as NtSOCS-1, NtSOCS-2 and NtSOCS-3. Multiple alignments showed that, the three SOCS molecules share highly conserved functional domains, including the SRC homology 2 (SH2) domain, the extended SH2 subdomain (ESS) and the SOCS box with others vertebrate counterparts. Phylogenetic analysis indicated that NtSOCS-1, 2 and 3 belong to the SOCS type II subfamily. Whereas NtSOCS-1 and 3 showed close evolutionary relationship with Perciformes, NtSOCS-2 was more related to Salmoniformes. Tissue specific expression results showed that, NtSOCS-1, 2 and 3 were constitutively expressed in all nine tissues examined. NtSOCS-1 and 3 were highly expressed in immune-related tissues, such as gills, foregut and head kidney. However, NtSOCS-2 was superlatively expressed in liver, brain and heart. In vivo, NtSOCS-1 and 3 mRNA levels were up-regulated after lipopolysaccharide (LPS) challenge while NtSOCS-2 was down-regulated. In vitro, LPS stimulation increased NtSOCS-3 mRNA expression, however it inhibited the transcription of NtSOCS-1 and 2. Collectively, our findings suggest that, the NtSOCS-1 and 3 might play significant role(s) in innate immune response, while NtSOCS-2 may be more involved in metabolic regulation.
dc.identifierLiu, C.Z., He, A.Y., Chen, L.Q., Limbu, S.M., Wang, Y.W., Zhang, M.L. and Du, Z.Y., 2016. Molecular characterization and immune response to lipopolysaccharide (LPS) of the suppressor of cytokine signaling (SOCS)-1, 2 and 3 genes in Nile tilapia (Oreochromis niloticus). Fish & shellfish immunology, 50, pp.160-167.
dc.identifierhttp://hdl.handle.net/20.500.11810/2690
dc.identifier10.1016/j.fsi.2016.01.027
dc.languageen
dc.publisherElsevier
dc.subjectNile tilapia (Oreochromis niloticus)
dc.subjectSuppressor of cytokine signaling
dc.subjectLPS
dc.subjectImmune regulation
dc.titleMolecular Characterization and Immune Response to Lipopolysaccharide (LPS) of the Suppressor of Cytokine Signaling (SOCS)-1, 2 And 3 Genes in Nile Tilapia (Oreochromis Niloticus)
dc.typeJournal Article, Peer Reviewed

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Department of Aquatic Sciences and Fisheries Technology