Existence of multiple ESBL genes among phenotypically confirmed ESBL producing klebsiella pneumoniae and escherichia coli concurrently isolated from clinical, colonization and contamination samples from neonatal units at Bugando Medical Center, Mwanza, Tanzania

Simple item page
dc.creatorSilago, Vitus
dc.creatorKovacs, Dory
dc.creatorSamson, Happyness
dc.creatorSeni, Jeremiah
dc.creatorMatthews, Louise
dc.creatorOravcová, Katarina
dc.creatorLupindu, Athumani M.
dc.creatorHoza, Abubakar S.
dc.creatorMshana, Stephen E.
dc.date.accessioned2021-04-29T09:09:11Z
dc.date.accessioned2025-08-05T07:21:00Z
dc.date.available2021-04-29T09:09:11Z
dc.date.created2021-04-29T09:09:11Z
dc.date.issued2021
dc.description.abstractThe proportions and similarities of extended-spectrum β-lactamase (ESBL) producing K. pneumoniae (ESBL-KP) and E. coli (ESBL-EC) carrying multiple ESBL genes is poorly known at our setting. This study investigated the existence of multiple ESBL genes (blaCTX-M, blaTEM, and blaSHV) among ESBL-KP and ESBL-EC concurrently isolated from clinical, colonization, and contamination samples from neonatology units in Mwanza-Tanzania. Twenty and 55 presumptive ESBL-EC and ESBL-KP, respectively, from a previous study archived at−80 ◦C were successfully recovered for this study. Isolates were screened and confirmed for production of ESBLs by phenotypic methods followed by multiplex PCR assay to determine ESBL genes. All (100%) and 97.3% of presumptive ESBL isolates were phenotypically confirmed by Clinical and Laboratory Standards Institute (CLSI) and modified double-disc synergy methods, respectively. About 93.3% (70/75) of phenotypically confirmed ESBL isolates had at least one ESBL gene, whereby for 62.9% (44/70), all ESBL genes (blaCTX-M, blaTEM, and blaSHV) were detected. Eight pairs of ESBL bacteria show similar patterns of antibiotics susceptibility and ESBL genes. ESBL-KP and ESBL-EC, concurrently isolated from clinical, colonization and contamination samples, harbored multiple ESBL genes. Further, eight pairs of ESBL isolates had similar patterns of antibiotics susceptibility and ESBL genes, suggesting transmission of and/or sharing of mobile genetic elements (MGEs) among ESBL-KP and ESBL-EC.
dc.identifierSilago, V.; Kovacs, D.; Samson, H.; Seni, J.; Matthews, L.; Oravcová, K.; Lupindu, A.M.; Hoza, A.S.; Mshana, S.E. Existence of Multiple ESBL Genes among Phenotypically Confirmed ESBL Producing Klebsiella pneumoniae and Escherichia coli Concurrently Isolated from Clinical, Colonization and Contamination Samples from Neonatal Units at Bugando Medical Center, Mwanza, Tanzania. Antibiotics 2021, 10, 476. https://doi.org/ 10.3390/antibiotics10050476
dc.identifierhttps://www.suaire.sua.ac.tz/handle/123456789/3497
dc.identifier.urihttp://repository.costech.or.tz/handle/20.500.14732/97115
dc.languageen
dc.publisherMDPI
dc.subjectAntimicrobial resistance
dc.subjectExtended-spectrum beta-lactamase
dc.subjectEscherichia coli
dc.subjectKlebsiella pneumoniae
dc.titleExistence of multiple ESBL genes among phenotypically confirmed ESBL producing klebsiella pneumoniae and escherichia coli concurrently isolated from clinical, colonization and contamination samples from neonatal units at Bugando Medical Center, Mwanza, Tanzania
dc.typeArticle

Files

Collections

Sokoine University