Comparative evaluation of different methods of extraction and purification used in technical enzyme production from microorganisms

Simple item page
dc.creatorGidamis, A. B.
dc.creatorNnko, A.
dc.creatorShayo, N. B.
dc.creatorChove, B. E.
dc.creatorKroner, H. K.
dc.date2017-06-28T11:20:40Z
dc.date2017-06-28T11:20:40Z
dc.date1998
dc.date.accessioned2022-10-25T08:50:17Z
dc.date.available2022-10-25T08:50:17Z
dc.descriptionTanzania Journal of Agricultural Sciences, 1998; I (2): 141-148
dc.descriptionA stlldy was conducted to compare the efficiency of two methods of microbial cell separation and enzyme purification using penicillin-G-acylase (PGA) from Escherichia coli. The efficiency of two methods of ceJ[ separation; centrifugation and cross flow filtration (CFF) were compared. The CFF method was found to have both higher separation efficiency and enzyme yield than centrifugation method. Centrifugation method gave a separation efficiency of 98.5% with enzyme yield of94 % whereas CFFmethod resulted in 100% separation efficiency and enzyme yield of98.8%. The Escherichia coli cells were disrupted by high pressure homogenization (HPH),and the disrupted cells were purified using two different techniques. Technique I was a combination of cross-flow-diafiltration (CFD) , ultrafiltration (UF) and heat/pH-shift treatments. This technique resulted in 47% enzyme yield with a purification factor of 12. Technique II which involved two extraction steps by' aqueous two - phase system (APS) coupled with UF resulted in 62 % enzyme yield with a pu;ificationfactor of 4. Technique I was therefore much better than techriique Il in purifying the enzyme. For higher enzyme yield, technique II would seem to be a better one than technique I.
dc.formatapplication/pdf
dc.identifierhttps://www.suaire.sua.ac.tz/handle/123456789/1751
dc.identifier.urihttp://hdl.handle.net/123456789/90020
dc.languageen
dc.publisherTanzania J. Agric. Sc
dc.subjectEnzyme yield
dc.subjectMicrobial enzymes
dc.subjectPurification factor
dc.subjectRecovery rate
dc.subjectSeparation efficiency
dc.subjectTechnical enzyme
dc.titleComparative evaluation of different methods of extraction and purification used in technical enzyme production from microorganisms
dc.typeArticle

Files

Collections

Sokoine University