In vivo antimycobacterial studies of toussaintine A-chitosan nanocomposites
| dc.creator | Rwegasila, Edward | |
| dc.creator | Munissi, Joan J.E | |
| dc.creator | Mubofu, Egid B. | |
| dc.creator | Nyandoro, Stephen S. | |
| dc.creator | Erasto, Paul | |
| dc.date | 2018-11-08T10:09:52Z | |
| dc.date | 2018-11-08T10:09:52Z | |
| dc.date | 2018-06 | |
| dc.date.accessioned | 2021-05-03T13:19:56Z | |
| dc.date.available | 2021-05-03T13:19:56Z | |
| dc.description | Chitosan (CS, molecular weight (MW) 20.2 kDA, stability of 210 °C and degree of deacetylation (DD) 73.31%) was obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the studied bioactive natural product, toussaintine A (TA) isolated from the leaves of Toussaintia orientalis, on a chitosan-tripolyphosphate (CS/TPP) nanoformulation was attained through ionotropic gelation. Characterization of pure CS, CS/TPP and TA-CS/TPP nanocomposites was carried out by FTIR and SEM. The encapsulation efficiency and loading capacity of the TA were 69.33 and 0.46%, respectively. The in vitro release kinetics established an initial release of 27% of TA in the initial six hours followed by a slow and maintained release up to 72 h. The in vivo antimycobacterial acitivities of both TA and TA-CS/TPP nanocomposites against Mycobacterium indicus pranii (MIP) employing Galleria mellonella larvae as an infection model were evaluated. TA-CS/TPP nanocomposite formulations exhibited remarkable effectiveness against MIP than free TA | |
| dc.description | International Foundation for Science (IFS) | |
| dc.identifier | Edward Rwegasila, Joan J.E. Munissi, Egid B. Mubofu, Stephen S. Nyandoro and Paul Erasto, In vivo antimycobacterial studies of toussaintine A-chitosan nanocomposites, Tanzania Journal of Science. 2018, 44 (2), 16 - 26. | |
| dc.identifier | 0856-1761 | |
| dc.identifier | http://hdl.handle.net/20.500.11810/4965 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.11810/4965 | |
| dc.language | en | |
| dc.publisher | Dar es Salaam University Press | |
| dc.subject | Toussaintine A; chitosan; nanocomposites, antimycobacterial; Galleria mellonella | |
| dc.title | In vivo antimycobacterial studies of toussaintine A-chitosan nanocomposites | |
| dc.type | Journal Article |