Molecular cloning and functional characterization of elongase (elovl5) and fatty acyl desaturase (fads2) in sciaenid, Nibea diacanthus (Lacepède, 1802)

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dc.creatorLin, Zhideng
dc.creatorZou, Weiguang
dc.creatorHuang, Yisheng
dc.creatorSamwel Mchele Limbu
dc.creatorHan, Fenglu
dc.creatorWen, Xiaobo
dc.date2019-05-08T05:41:10Z
dc.date2019-05-08T05:41:10Z
dc.date2019-02-10
dc.date.accessioned2021-05-07T07:47:50Z
dc.date.available2021-05-07T07:47:50Z
dc.descriptionIn the present paper, we investigated the molecular cloning and functional characterization of elongase of very long chain fatty acid (elovl) and fatty acyl desaturase (fads) genes in a marine teleost, Nibea diacanthus. The elongase cDNA sequence encoded a polypeptide of 294 amino acids exhibiting Elovl5 activity, which effectively elongated both C18 (18:2n−6, 18:3n−3 and 18:3n−6) and C20 (20:4n−6 and 20:5n−3) polyunsaturated fatty acids. The desaturase cDNA sequence specified a polypeptide of 445 amino acids indicating Δ6 desaturation activity, which coul converted C18:2n−6 and C18:3n−3 to C18:3n−6 and C18:4n−3, respectively. Tissue distribution analysis by quantitative real-time PCR revealed that the elovl5 was primarily expressed in intestine and liver, while the fads2 was mainly expressed in liver and brain. These results increase our knowledge of the ability of endogenous highly unsaturated fatty acids (HUFA) biosynthesis in marine carnivorous fish.
dc.descriptionGuangdong Oceanic and Fishery Science and Technology Foundation (No. A201005D06-1).
dc.identifierhttp://hdl.handle.net/20.500.11810/5237
dc.identifierhttps://doi.org/10.1016/j.gene.2019.01.033.
dc.identifier.urihttp://hdl.handle.net/20.500.11810/5237
dc.publisherElsevier
dc.subjectElovl5
dc.subjectFads2
dc.subjectHUFA
dc.subjectNibea diacanthus
dc.titleMolecular cloning and functional characterization of elongase (elovl5) and fatty acyl desaturase (fads2) in sciaenid, Nibea diacanthus (Lacepède, 1802)
dc.typeJournal Article, Peer Reviewed

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Department of Aquatic Sciences and Fisheries Technology