CD5+ B lymphocytes are the main source of antibodies reactive with non-parasite antigens in Trypanosoma congolense-infected cattle
| dc.creator | Buza, Joram | |
| dc.creator | Sileghem, Maarten | |
| dc.creator | Gwakisa, Paul | |
| dc.creator | Naessens, Jan | |
| dc.date | 2019-10-08T06:30:41Z | |
| dc.date | 2019-10-08T06:30:41Z | |
| dc.date | 1997 | |
| dc.date.accessioned | 2022-10-25T09:20:45Z | |
| dc.date.available | 2022-10-25T09:20:45Z | |
| dc.description | Research Article published by Blackwell Science Ltd | |
| dc.description | Mice infected with African trypanosomes produce exceptionally large amounts of serum IgM, a major part of which binds to non-trypanosome antigens such as trinitrophenol and single-strand DNA. In this paper, we describe that in cattle infected with Trypanosoma congolense and T. vivax, similar antibodies are found, although they bind mainly to protein antigens, such as b-galactosidase, ovalbumin and ferritin. The parasite non-specific IgM antibodies appear around the same time as the parasite-specific antibodies, but their origin and function are not clear.We tested the hypothesis that CD5+ B cells (or B-1 cells), which increase during trypanosome infections in cattle, are responsible for production of antibodies to non-trypanosome antigens. Splenic CD5+ and CD5− B cells from infected cattle were sorted and tested in a single cell blot assay. The numbers of immunoglobulin-secreting cells were similar in both B-cell populations. However, antibodies with reactivity for non-trypanosome antigens were significantly more prevalent in the CD5+ B-cell fraction and were exclusively IgM. The preference for production of these antibodies by CD5+ B cells and the expansion of this subpopulation during infections in cattle, strongly suggest that CD5+ B cells are the main source of trypanosome non-specific antibodies. We propose that these antibodies are natural, polyreactive antibodies that are predominantly secreted by CD5+ B cells. Since B-1 cells are up-regulated in many states of immune insufficiency, the immunosuppression associated with trypanosome infections may be responsible for the increase of this subset and the concomitant increase in trypanosome non-specific antibodies. | |
| dc.format | application/pdf | |
| dc.identifier | http://dspace.nm-aist.ac.tz/handle/123456789/463 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/95205 | |
| dc.language | en | |
| dc.publisher | Blackwell Science Ltd | |
| dc.subject | Research Subject Categories::NATURAL SCIENCES | |
| dc.title | CD5+ B lymphocytes are the main source of antibodies reactive with non-parasite antigens in Trypanosoma congolense-infected cattle | |
| dc.type | Article |