Induction of Early Apoptosis and Reactive Oxygen Species (ROS) Production by Tanzanian Basidiomycete ( Cantharellus Miomboensis )

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dc.creatorMasalu, Rose
dc.creatorHosea, Ken M.
dc.creatorMeyer, Mervin
dc.creatorLyantagaye, Sylvester L.
dc.creatorKanyande, Stonard
dc.date2016-06-26T17:30:48Z
dc.date2016-06-26T17:30:48Z
dc.date2010
dc.date.accessioned2018-03-27T09:00:36Z
dc.date.available2018-03-27T09:00:36Z
dc.descriptionCantharellus miomboensis is a new basidiomycete fungus recently found in Miombo woodlands in Tanzania. In this study, crude extract was prepared from fruiting bodies of C. miomboensis and was in vitro screened for its cytotoxicity using Tetrazolium salt (3-(4,5-dimethlthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) in human cell lines namely; Hepatocellular carcinoma (HepG2), Human non-small cell lung carcinoma (H157) and Human colon adenocarcinoma (HT.29). Thereafter, pro-apoptotic effects were determined using biochemical changes in apoptotic cells. These included externalization of phospholipid phosphatidylserine (PS) using APO Percentage dye by flow cytometry and depolarization of mitochondrial membrane potential using Tetramethyl rhodamine ethyl ester perchlorate (TMRE) assay. The test extract was found to induce dose dependent PS externalization on human cell lines when treated with various concentrations (1 - 5 mg/ml) and completely depolarized the mitochondrial membrane potential after 6 hours on HepG2 cell line. When the extract was examined for ROS production using 2’,7’- dichlorofluorescin diacetate (DCFH-DA) staining, there was no ROS generation found in HepG2 cells. It is therefore concluded that C. miomboensis extract is able to induce apoptosis in HepG2 cells and PS externalization and loss of mitochondrial membrane potential in HepG2 cells appear to be independent of ROS production.
dc.identifierMasalu, R., Hosea, K.M., Meyer, M., Lyantagay, S. and Kanyande, S., 2010. Induction of early apoptosis and reactive oxygen species (ROS) production by Tanzanian basidiomycete (Cantharellus miomboensis). International Journal of Biological and Chemical Sciences, 4(4).
dc.identifier1991-8631
dc.identifierhttp://hdl.handle.net/20.500.11810/2759
dc.identifier10.4314/ijbcs.v4i4.62955
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2759
dc.languageen
dc.subjectMiombo woodlands
dc.subjectApoptosis
dc.subjectBasidiomycete
dc.subjectReactive oxygen species
dc.subjectCarcinoma
dc.titleInduction of Early Apoptosis and Reactive Oxygen Species (ROS) Production by Tanzanian Basidiomycete ( Cantharellus Miomboensis )
dc.typeJournal Article, Peer Reviewed

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