Toxigenic Vibrio Cholerae Identified in Estuaries of Tanzania Using PCR Techniques

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dc.creatorDalusi, Lucy
dc.creatorLyimo, Thomas J.
dc.creatorLugomela, Charles
dc.creatorHosea, Ken M.
dc.creatorSjöling, Sara
dc.date2016-06-18T18:14:26Z
dc.date2016-06-18T18:14:26Z
dc.date2015
dc.date.accessioned2018-03-27T08:23:06Z
dc.date.available2018-03-27T08:23:06Z
dc.descriptionThe current study assessed the occurrence of the Vibrio cholerae serogroups O1 and O139 in environmental samples along salinity gradients in three selected estuaries of Tanzania both through culture independent methods and by cultured bacteria. Occurrence of V. cholerae was determined by PCR targeting the V. cholerae outer membrane protein gene ompW. Furthermore, the presence of toxigenic strains and serogroups O1 and O139 was determined using multiplex PCR with specific primers targeting the cholera toxin gene subunit A, ctxA, and serotype specific primers, O1-rfb and O139-rfb, respectively. Results showed that V. cholerae occurred in approximately 10% (n = 185) of both the environmental samples and isolated bacteria. Eight of the bacteria isolates (n = 43) were confirmed as serogroup O1 while one belonged to serogroup O139, the first reported identification of this epidemic strain in East African coastal waters. All samples identified as serogroup O1 or O139 and a number of non-O1/O139 strains were ctxA positive. This study provides in situ evidence of the presence of pathogenic V. cholerae O1 and O139 and a number of V. cholerae non-O1/O139 that carry the cholera toxin gene in estuaries along the coast of Tanzania.
dc.identifierDalusi, L., Lyimo, T.J., Lugomela, C., Hosea, K.M. and Sjöling, S., 2015. Toxigenic Vibrio cholerae identified in estuaries of Tanzania using PCR techniques. FEMS microbiology letters, 362(5), p.fnv009.
dc.identifierhttp://hdl.handle.net/20.500.11810/2580
dc.identifier10.1093/femsle/fnv009
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2580
dc.languageen
dc.subjectCholera
dc.subjectestuaries
dc.subjectVibrio cholerae
dc.subjectSediment
dc.subjectTanzania
dc.subjectEast Africa
dc.titleToxigenic Vibrio Cholerae Identified in Estuaries of Tanzania Using PCR Techniques
dc.typeJournal Article

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Department of Aquatic Sciences and Fisheries Technology