Two-Stage Fungal Pre-Treatment for Improved Biogas Production from Sisal Leaf Decortication Residues
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Description
Sisal leaf decortications residue (SLDR) is amongst the most abundant agroindustrial
residues in Tanzania and is a good feedstock for biogas production. Pretreatment
of the residue prior to its anaerobic digestion (AD) was investigated using a twostage
pre-treatment approach with two fungal strains, CCHT-1 and Trichoderma reesei in
succession in anaerobic batch bioreactors. AD of the pre-treated residue with CCTH-1 at
10% (wet weight inoculum/SLDR) inoculum concentration incubated for four days
followed by incubation for eight days with 25% (wet weight inoculum/SLDR) of T. reesei
gave a methane yield of 0.292 ± 0.04 m3 CH4/kg volatile solids (VS)added. On reversing the
pre-treatment succession of the fungal inocula using the same parameters followed by AD,
methane yield decreased by about 55%. Generally, an increment in the range of 30–101%
in methane yield in comparison to the un-treated SLDR was obtained. The results
confirmed the potential of CCHT-1 followed by Trichoderma reesei fungi pre-treatment
prior to AD to achieve significant improvement in biogas production from SLDR.
Keywords
Two-stage pre-treatment, Sisal leaf decortications residue, Biological, Fungi, Lignocellulose, Anaerobic digestion