Comparative evaluation of different methods of extraction and purification used in technical enzyme production from microorganisms

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dc.creatorGidamis, A.B.
dc.creatorNnko, S.A.
dc.creatorShayo, N.B.
dc.creatorChove, B.E.
dc.creatorKroner, H. K.
dc.date2017-12-08T11:27:35Z
dc.date2017-12-08T11:27:35Z
dc.date1998
dc.date.accessioned2022-10-25T08:50:59Z
dc.date.available2022-10-25T08:50:59Z
dc.descriptionTanzania Journal of Agriculture Science 1998, Vol. 1(2), pp 141-148
dc.descriptionA stlldy was conducted to compare the efficiency of two methods of microbial cell separation and enzyme purification using penicillin-G-acylase (PGA) from Escherichia coli. The efficiency of two methods of ceJ[ separation; centrifugation and cross flow filtration (CFF) were compared. The CFF method was found to have both higher separation efficiency and enzyme yield than centrifugation method. Centrifugation method gave a separation efficiency of 98.5% with enzyme yield of94 % whereas CFFmethod resulted in 100% separation efficiency and enzyme yield of98.8%. The Escherichia coli cells were disrupted by high pressure homogenization (HPH),and the disrupted cells were purified using two different techniques. Technique I was a combination of cross-flow-diafiltration (CFD) , ultrafiltration (UF) and heat/pH-shift treatments. This technique resulted in 47% enzyme yield with a purification factor of 12. Technique II which involved two extraction steps by' aqueous two - phase system (APS) coupled with UF resulted in 62 % enzyme yield with a pu;ificationfactor of 4. Technique I was therefore much better than techriique Il in purifying the enzyme. For higher enzyme yield, technique II would seem to be a better one than technique I.
dc.formatapplication/pdf
dc.identifierhttps://www.suaire.sua.ac.tz/handle/123456789/1845
dc.identifier.urihttp://hdl.handle.net/123456789/90896
dc.languageen
dc.subjectMicrobial enzymes
dc.subjectPurification factor
dc.subjectRecovery rate
dc.subjectSeparation efficiency
dc.subjectTechnical enzyme
dc.titleComparative evaluation of different methods of extraction and purification used in technical enzyme production from microorganisms
dc.typeArticle

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