Curdlan-Conjugated PLGA Nanoparticles Possess Macrophage Stimulant Activity and Drug Delivery Capabilities

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dc.creatorTukulula, Matshawandile
dc.creatorHayeshi, Rose
dc.creatorFonteh, Pascaline
dc.creatorMeyer, Debra
dc.creatorNdamase, Abongile
dc.creatorMadziva, Michael T.
dc.creatorKhumalo, Vincent
dc.creatorLubuschagne, Philip
dc.creatorNaicker, Brendon
dc.creatorSwai, Hulda
dc.creatorDube, Admire
dc.date2019-10-18T07:52:58Z
dc.date2019-10-18T07:52:58Z
dc.date2015-03-28
dc.date.accessioned2022-10-25T09:21:02Z
dc.date.available2022-10-25T09:21:02Z
dc.descriptionResearch Article published by Springer
dc.descriptionPurpose There is significant interest in the application of nanoparticles to deliver immunostimulatory signals to cells.We hypothesized that curdlan (immune stimulating polymer) could be conjugated to PLGA and nanoparticles from this copolymer would possess immunostimulatory activity, be non-cytotoxic and function as an effective sustained drug release system. Methods Carbodiimide chemistry was employed to conjugate curdlan to PLGA. The conjugate (C-PLGA) was characterized using 1H and 13C NMR, FTIR, DSC and TGA. Nanoparticles were synthesized using an emulsion-solvent evaporation technique. Immunostimulatory activity was characterized in THP-1 derived macrophages. MTTassay and real-time impedance measurements were used to characterize polymer and nanoparticle toxicity and uptake in macrophages. Drug delivery capability was assessed across Caco-2 cells using rifampicin as a model drug. Results Spectral characterization confirmed successful synthesis of C-PLGA. C-PLGA nanoparticles enhanced phosphorylated ERK production in macrophages indicating cell stimulation. Nanoparticles provided slow release of rifampicin across Caco-2 cells. Polymers but not nanoparticles altered the adhesion profiles of the macrophages. Impedance measurements suggested Ca2+ dependent uptake of nanoparticles by the macrophages. Conclusions PLGA nanoparticles with macrophage stimulating and sustained drug delivery capabilities have been prepared. These nanoparticles can be used to stimulate macrophages and concurrently deliver drug in infectious disease therapy.
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dc.identifierDOI 10.1007/s11095-015-1655-9
dc.identifierhttp://dspace.nm-aist.ac.tz/handle/123456789/504
dc.identifier.urihttp://hdl.handle.net/123456789/95378
dc.languageen
dc.publisherSpringer
dc.subjectimmunostimulantnanoparticles
dc.subjectreal-time impedance measurements
dc.subjectPLGA nanoparticles
dc.titleCurdlan-Conjugated PLGA Nanoparticles Possess Macrophage Stimulant Activity and Drug Delivery Capabilities
dc.typeArticle

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