Oxalic Acid Pretreatment, Fungal Enzymatic Saccharification and Fermentation of Maize Residues to Ethanol
| dc.creator | Mtui, Godliving Y. S. | |
| dc.date | 2016-09-21T14:18:06Z | |
| dc.date | 2016-09-21T14:18:06Z | |
| dc.date | 2011-12 | |
| dc.date.accessioned | 2018-03-27T09:00:47Z | |
| dc.date.available | 2018-03-27T09:00:47Z | |
| dc.description | Dilute oxalic acid pretreatment was investigated as a feasible method for conversion of maize residues into sugars and subsequent production of bioethanol. Oxalic acid concentration of 200 mM for samples treated at 160°C for 10 min was found to be the best pretreatment conditions. Cellulases, mainly cellobiohydrolases from Trichoderma reesei, recorded activities of up to 116 U/ml during saccharification of the cellulosic substrates. The optimum saccharification conditions using 0.2% (v/v) enzyme concentrate were the incubation temperature of 45°C for 48 h. Up to 46% (w/w) glucose and 28% (w/w) xylose were obtained from the pretreated maize residues (stalk, leaf, husk and cobs). Furthermore, a native Saccharomyces cerevisiae (strain KB) was able to convert 78% (w/w) of glucose (and other fermentable sugars) to ethanol after 60 h of incubation at 32°C, under stationary culture conditions. The challenges encountered in chemical and biological conversions included incomplete hydrolysis and fermentation due to substrate recalcitrance and the inability of the yeast cells to utilize 5-carbon sugars such as xylose. This study therefore provided baseline research data and information that could be used for more elaborate and scaled-up studies for possible industrial-scale conversion of the readily available crop residues into bioenergy. | |
| dc.identifier | Mtui, G.Y., 2012. Oxalic acid pretreatment, fungal enzymatic saccharification and fermentation of maize residues to ethanol. African Journal of Biotechnology, 11(4), pp.843-851. | |
| dc.identifier | org/10.5897/AJB11.3032 | |
| dc.identifier | http://hdl.handle.net/20.500.11810/4021 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.11810/4021 | |
| dc.language | en | |
| dc.subject | Bioethanol, cellulases | |
| dc.subject | Cellulases | |
| dc.subject | Fermentation | |
| dc.subject | Hydrolysates | |
| dc.subject | Maize residues | |
| dc.subject | Oxalic acid | |
| dc.subject | Pretreatment | |
| dc.subject | Saccharification | |
| dc.title | Oxalic Acid Pretreatment, Fungal Enzymatic Saccharification and Fermentation of Maize Residues to Ethanol | |
| dc.type | Journal Article, Peer Reviewed |