Optimization of Laccase and Manganese Peroxidase Production in Submerged Culture of Pleurotus Sajor-caju

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dc.creatorPatrick, Ferdinandi
dc.creatorMtui, Godliving
dc.creatorMligo, Anthony M.
dc.creatorKivaisi, Amelia K.
dc.date2016-05-17T13:28:53Z
dc.date2016-05-17T13:28:53Z
dc.date2013
dc.date.accessioned2018-03-27T09:00:18Z
dc.date.available2018-03-27T09:00:18Z
dc.descriptionA white-rot fungus, Pleurotus sajor-caju, was isolated from coastal Tanzania and screened for crude lignolytic enzymes production using rhemazol brilliant blue R (RBBR) dye, 2,2-azino-bis (3- ethylbenzthiazoline)-6-sulfonate (ABTS) and guaiacol in a semi-solid medium. Laccase (Lac) and manganese peroxidase (MnP) were detected by α-napthol and pyrogallol solutions, respectively, on the guaiacol supplemented semi-solid media. The effect of temperature, pH, carbon, nitrogen, Cu2+, 2,5- xylidine, ferulic acid, Mn2+ and immobilization using Luffa cylindrica sponges in submerged culture fermentations were investigated for maximum enzymes production. After 7 days of incubation, 83 to 100% oxidation of RBBR, ABTS and guaiacol was observed. With optimized culture conditions, the fungal filtrate had maximum Lac and MnP activities of 80 and 0.94 U/ml, respectively compared to 0.62 and 0.0003 U/ml obtained with non-optimized ones; amounting to 129 and 3133 times increase in Lac and MnP activities, respectively. The improved crude enzymes activities, RBBR decolourization, ABTS and guaiacol oxidation capabilities of P. sajor-caju show its potential as a source of industrial enzymes for biotechnological applications.
dc.identifierPatrick, F., Mtui, G., Mshandete, A.M. and Kivaisi, A., 2013. Optimization of laccase and manganese peroxidase production in submerged culture of Pleurotus sajorcaju. African Journal of Biotechnology, 10(50), pp.10166-10177.
dc.identifier1684–5315
dc.identifierhttp://hdl.handle.net/20.500.11810/2096
dc.identifier10.5897/AJB11.863
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2096
dc.languageen
dc.subjectWhite-rot fungi
dc.subjectOptimization
dc.subjectPleurotus sajor-caju
dc.subjectLaccase
dc.subjectManganese peroxidase
dc.subjectSubmerged fermentation
dc.subjectImmobilization
dc.titleOptimization of Laccase and Manganese Peroxidase Production in Submerged Culture of Pleurotus Sajor-caju
dc.typeJournal Article, Peer Reviewed

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