Molecular characterization of African swine fever virus from domestic pigs in northern Tanzania during an outbreak in 2013

Misinzo, Gerald; Kwavi, David E; Sikombe, Christopher D; Makange, Mariam; Peter, Emma; Muhairwa, Amandus P; Madege, Michael J

dc.creator	Misinzo, Gerald
dc.creator	Kwavi, David E
dc.creator	Sikombe, Christopher D
dc.creator	Makange, Mariam
dc.creator	Peter, Emma
dc.creator	Muhairwa, Amandus P
dc.creator	Madege, Michael J
dc.date	2022-06-14T10:46:38Z
dc.date	2022-06-14T10:46:38Z
dc.date	2014-10
dc.date.accessioned	2022-10-25T08:50:05Z
dc.date.available	2022-10-25T08:50:05Z
dc.identifier	https://www.suaire.sua.ac.tz/handle/123456789/4257
dc.identifier.uri	http://hdl.handle.net/123456789/89837
dc.description	African swine fever (ASF) is an acute, highly contagious and deadly viral hemorrhagic fever of domestic pigs caused by African swine fever virus (ASFV), a double-stranded DNA virus of the family Asfarviridae. In this study, molecular diagnosis and characterization of outbreak ASFV in northern Tanzania, was performed on spleen, lymph node, kidney, and heart samples collected in June and July 2013 from domestic pigs that died during a hemorrhagic disease outbreak. Confirmatory diagnosis of ASF was performed using polymerase chain reaction (PCR) by partial amplification of B646L gene of ASFV encoding the major capsid protein p72 using PPA1/PPA2 primers. PCR using PPA1/PPA2 primers produced an expected PCR product size, confirming ASF outbreak in northern Tanzania. In addition, nucleotide amplification and sequencing, and phylogenetic reconstruction of the variable 3′-end of the …
dc.format	application/pdf
dc.language	en
dc.publisher	Springer Netherlands
dc.subject	Molecular characterization
dc.subject	Africa
dc.subject	swine fever virus
dc.subject	domestic pigs
dc.subject	northern Tanzania
dc.title	Molecular characterization of African swine fever virus from domestic pigs in northern Tanzania during an outbreak in 2013
dc.type	Article