Chikungunya is an arboviral disease transmitted by aedes mosquitoes, caused by Chikungungunya virus. It consists of an acute illness characterized by fever, rash, and incapacitating arthralgia. This study aimed to estimate the prevalence of Chikungunya fever in patients presenting fever at different health facilities located in Kyela district. Out of 132 recruited patients, 94(71.2 %) were female and 38 (28.8 %) were male. The majority of them 80 (60.6%) were adults (≥25 years). Anti-Chikungunya virus anti-immunoglobulin G (IgG) and anti-immunoglobulin M (IgM) antibodies were detected in serum samples using indirect enzyme-linked immunosorbent assay. Chikungunya virus IgG or IgM antibodies were detected in 19 among 132 serum specimens tested indicating a seroprevalence of 14.3%. Out of 132 sera tested, 14 (11%) had IgG antibodies and 5(3.8%) had IgM antibodies. The higher anti-CHIKV IgG seroprevalence was found in female patients (OR= 3.22; 95% CI: 1.03-10.06) than in male. Similarly patients who took some medication before going to the health centre were found with high CHIKV IgG antibodies (OR= 13.912; 95% CI: 1.76-109.78) as well as in patients who never been vaccinated (OR=4.6; 95%CI: 0.02 – 1.71). Additionally, the uni-variate analysis results revealed, feeling nausea as the symptom of significant association with Chikungunya IgG seropositivity (OR = 4.5; 95% CI: 1.3– 14.4). These findings confirm that CHIKV infection seems to be among the common causes of febrile illness in Kyela district and appears to be actively circulating in the population but is routinely misdiagnosed. This suggests a need to raise awareness among health facilities and policy makers on the use of specific diagnosis for better control of arbovirus diseases in the study region.