Journal of Advanced Veterinary Research Volume 9, Issue 2 (2019) 64-68African trypanosomes are etiological agents of trypanosomosis transmitted by tsetse flies (Glossina spp). Thus, identifying them in vectors and hosts together with their classification into species, sub species is crucial for effective control of the diseases they cause to animals and human. This study analysed 350 samples collected from cattle (100) and tsetse flies (250) of Meatu district for identification of trypanosomes through amplification of Internal transcribed spacer 1 (ITS-1) region in order to sup port formulation of tsetse and trypanosomosis control strategies within the district. Occurrence of try panosomes in cattle was 15%, while in tsetse was 1.20%. Trypanosoma congolense was identified in cattle whereas; T. simiae and T. godfreyi were identified in tsetse flies. The tsetse and trypanosomosis control options were discussed.