| dc.creator |
Mwanyika, Gaspary O. |
|
| dc.creator |
Mboera, Leonard E. G. |
|
| dc.creator |
Rugarabamu, Sima |
|
| dc.creator |
Makange, Mariam |
|
| dc.creator |
Sindato, Calvin |
|
| dc.creator |
Lutwama, Julius J. |
|
| dc.creator |
Paweska, Janusz T. |
|
| dc.creator |
Misinzo, Gerald |
|
| dc.date |
2022-09-12T07:07:37Z |
|
| dc.date |
2022-09-12T07:07:37Z |
|
| dc.date |
2021 |
|
| dc.date.accessioned |
2022-10-25T08:53:26Z |
|
| dc.date.available |
2022-10-25T08:53:26Z |
|
| dc.identifier |
http://www.suaire.sua.ac.tz/handle/123456789/4545 |
|
| dc.identifier.uri |
http://hdl.handle.net/123456789/93707 |
|
| dc.description |
Journal of Pathogens and global health |
|
| dc.description |
Dengue is an important mosquito-borne viral disease in humans in tropical and subtropical
countries. In 2019, a total of 6917 dengue cases were reported in Tanzania based on serological
analysis. The aim of this study was to confirm the presence of dengue virus (DENV) and conduct
its genetic characterization. A total of 191 serum samples were collected from the outpatients
seeking care from health facilities in Kinondoni and Ilala districts between March and May 2019.
All the samples were initially tested for the presence of non-structural protein 1 and anti-DENV
immunoglobulin G (IgG) and IgM using a commercial OnSite Duo Dengue Ag-IgG/IgM rapid
test. Of the 191 sera, 110 (57.6%) were DENV seropositive. The presence of DENV ribonucleic
acid was confirmed in 18.2% of the seropositive sera by reverse transcription polymerase chain
reaction (RT-PCR). The RT-PCR products were cleaned and partial sequences of DENV poly
protein gene determined using dideoxynucleotide cycle sequencing followed by phylogenetic
analysis. We present the occurrence of DENV serotype 1 (DENV-1) during the 2019 outbreak in
Tanzania. The DENV-1 strains reported in the present study are highly identical and cluster with
Asian DENV-1 strains indicating the possibility of intercontinental spread of DENV through
globalization. We advocate for the need for molecular surveillance of dengue viruses during
outbreaks to provide rapid evidence of the disease to guide public health interventions. |
|
| dc.format |
application/pdf |
|
| dc.language |
en |
|
| dc.publisher |
Informa UK Limited, trading as Taylor & Francis Group. |
|
| dc.subject |
Dengue virus |
|
| dc.subject |
Serotype |
|
| dc.subject |
Outbreak |
|
| dc.subject |
Tanzania |
|
| dc.title |
Circulation of dengue serotype 1 viruses during the 2019 outbreak in Dar es Salaam, Tanzania |
|
| dc.type |
Article |
|