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Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya.

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dc.creator Akoko, James
dc.creator Pelle, Roger
dc.creator Kivali, Velma
dc.creator Schelling, Esther
dc.creator Machuka, Eunice
dc.creator Mathew, Coletha
dc.creator Fèvre, Eric
dc.creator Kyallo, Victoria
dc.creator Falzon, Laura
dc.creator Lukambagire, Abdul
dc.creator Halliday, Jo
dc.creator Bonfoh, Bassirou
dc.creator Kazwala, Rudovick
dc.creator Ouma, Collins
dc.creator Shirima, Gabriel
dc.date 2020-06-12T08:38:17Z
dc.date 2020-06-12T08:38:17Z
dc.date 2020-05-11
dc.date.accessioned 2022-10-25T09:20:39Z
dc.date.available 2022-10-25T09:20:39Z
dc.identifier https://doi.org/10.1186/s12917-020-02346-y
dc.identifier https://dspace.nm-aist.ac.tz/handle/20.500.12479/792
dc.identifier.uri http://hdl.handle.net/123456789/95118
dc.description This research article published by BioMed Central Ltd, 2020
dc.description Background: Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results: A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RTPCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion: The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs.
dc.format application/pdf
dc.language en
dc.publisher BioMed Central Ltd
dc.subject Serology
dc.subject “Molecular detection”
dc.subject “Molecular evidence”
dc.subject “Pig brucellosis”
dc.title Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya.
dc.type Article


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