| dc.creator |
Omarch, Geofrey |
|
| dc.creator |
Kippie, Yunus |
|
| dc.creator |
Mentor, Shireen |
|
| dc.creator |
Ebrahim, Naushaad |
|
| dc.creator |
Fisher, David |
|
| dc.creator |
Murilla, Grace |
|
| dc.creator |
Swai, Hulda |
|
| dc.creator |
Dube, Admire |
|
| dc.date |
2019-10-18T07:42:13Z |
|
| dc.date |
2019-10-18T07:42:13Z |
|
| dc.date |
2019-04-22 |
|
| dc.date.accessioned |
2022-10-25T09:20:48Z |
|
| dc.date.available |
2022-10-25T09:20:48Z |
|
| dc.identifier |
https://doi.org/10.1080/21691401.2019.1596923 |
|
| dc.identifier |
http://dspace.nm-aist.ac.tz/handle/123456789/503 |
|
| dc.identifier.uri |
http://hdl.handle.net/123456789/95253 |
|
| dc.description |
Research Article published by Taylor & Francis Group VOL. 47, NO. 1, 2019 |
|
| dc.description |
Nanoparticles (NPs) have gained importance in addressing drug delivery challenges across biological
barriers. Here, we reformulated pentamidine, a drug used to treat Human African Trypanosomiasis
(HAT) in polymer based nanoparticles and liposomes and compared their capability to enhance pentamidine
penetration across blood brain barrier (BBB). Size, polydispersity index, zeta potential, morphology,
pentamidine loading and drug release profiles were determined by various methods.
Cytotoxicity was tested against the immortalized mouse brain endothelioma cells over 96 h. Moreover,
cells monolayer integrity and transportation ability were examined for 24 h. Pentamidine-loaded polycaprolactone
(PCL) nanoparticles had a mean size of 267.58, PDI of 0.25 and zeta potential of –28.1 mV
and pentamidine-loaded liposomes had a mean size of 119.61 nm, PDI of 0.25 and zeta potential
11.78. Pentamidine loading was 0.16 mg/mg (w/w) and 0.17 mg/mg (w/w) in PCL NPs and liposomes
respectively. PCL nanoparticles and liposomes released 12.13% and 22.21% of pentamidine respectively
after 24 h. Liposomes transported 87% of the dose, PCL NPs 66% of the dose and free pentamidine
penetration was 63% of the dose. These results suggest that liposomes are comparatively promising
nanocarriers for transportation of pentamidine across BBB. |
|
| dc.format |
application/pdf |
|
| dc.language |
en |
|
| dc.publisher |
Taylor & Francis Group. |
|
| dc.subject |
transendothelial electrical resistance |
|
| dc.subject |
Human African Trypanosomiasis |
|
| dc.subject |
blood brain barrier |
|
| dc.title |
Comparative in vitro transportation of pentamidine across the blood-brain barrier using polycaprolactone nanoparticles and phosphatidylcholine liposomes |
|
| dc.type |
Article |
|