Livestock grazing is an anthropogenic disturbance on the soil properties in montane forests of the northern slopes of Mount Kilimanjaro. It was determined the effect of livestock grazing on SOC, N, CEC, SAS, SFD and P in the montane forest. Forty plots were randomly established for collection of soil samples. Data showed livestock grazing decreased the accumulation of SOC and consequently negatively affected the natural pattern of N, P, SFD, CEC and SAS. An opposing pattern of available soil phosphorus was interpreted that the nutrient pool retained in plant tissues particularly in the long-lived trees, which were absent in heavily grazed area. The observed positive correlation between SOC with TN and CEC implies the livestock grazing impacts on the former negatively affected the later soil parameters. Low fungal density in degraded parts of the montane forest indicates that they are sensitive to the current land use change. Based on the observation from this study, it can be regarded that excessive livestock grazing has a long-term negative ramification for soil conservation in montane forest. An important decision has to be sought off with regard to the conservation management of soil characteristics of the montane forest in Kilimanjaro Mountain.