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Comparison of Escherichia Coli KO11 and Saccharomyces Cerevisiae ATCC 96581 in Fermenting Pinus Patula Hydrolysate Pretreated at Different Steam Explosion Severity

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dc.creator Chacha, Nyangi
dc.creator Dyrset, Nils
dc.creator Mtui, Godliving
dc.creator Katima, Jamidu H. Y.
dc.creator Mrema, Godwill
dc.date 2016-05-27T12:36:42Z
dc.date 2016-05-27T12:36:42Z
dc.date 2012
dc.date.accessioned 2018-03-27T08:41:05Z
dc.date.available 2018-03-27T08:41:05Z
dc.identifier Chacha, N., Dyrset, N., Mtui, G., Katima, J. and Mrema, G., 2012. Comparison of Escherichia coli KO11 and Saccharomyces cerevisiae ATCC 96581 in fermenting Pinus patula hydrolysate pretreated at different steam explosion severity. African Journal of Biotechnology, 11(37), p.9054.
dc.identifier http://hdl.handle.net/20.500.11810/2290
dc.identifier 10.5897/AJB11.3498
dc.identifier.uri http://hdl.handle.net/20.500.11810/2290
dc.description Bioconversion of wood residues to biofuels such as ethanol is one of the feasible initiatives towards production of renewable energy. This work compares the effectiveness of Escherichia coli KO11 and Saccharomyces cerevisiae ATCC 96581 in fermenting Pinus patula (pine) hydrolysate pretreated by acid-catalyzed steam explosion. The results show no significant difference in terms of the ethanol yield when E. coli KO11 and S. cerevisiae ATCC 96581 were used. The maximum ethanol concentration obtained in test tubes fermentation were 18.30 and 19.41 g/l for E. coli KO11 and S. cerevisiae ATCC 96581, respectively, from samples pretreated at 225°C/5 min. The ethanol yields obtained using bioreactors for samples pretreated at 225°C, 0.5% SO2, and 5 min, were 21.30 and 19.63 g/l for E. coli and S. cerevisiae, respectively. Overall, ethanol yields were higher in bioreactors than in test tubes for both strains. In comparison, S. cerevisiae consumed the substrate faster than E. coli, thus making S. cerevisiae the most preferred strain in fermentation of hydrolysates from steam pretreated P. patula.
dc.language en
dc.subject Pinus patula
dc.subject Steam explosion pretreatment
dc.subject Enzymatic hydrolysis
dc.subject Fermentation
dc.subject Escherichia coli
dc.subject Saccharomyces cerevisiae
dc.title Comparison of Escherichia Coli KO11 and Saccharomyces Cerevisiae ATCC 96581 in Fermenting Pinus Patula Hydrolysate Pretreated at Different Steam Explosion Severity
dc.type Journal Article, Peer Reviewed


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