| dc.creator |
Mbukwa, Elbert A. |
|
| dc.creator |
Msagati, Titus A. M. |
|
| dc.creator |
Mamba, Bhekie B. |
|
| dc.date |
2016-06-15T20:52:57Z |
|
| dc.date |
2016-06-15T20:52:57Z |
|
| dc.date |
2012 |
|
| dc.date.accessioned |
2018-03-27T08:54:31Z |
|
| dc.date.available |
2018-03-27T08:54:31Z |
|
| dc.identifier |
Mbukwa, E.A., Msagati, T.A. and Mamba, B.B., 2012. Quantitative variations of intracellular microcystin-LR,-RR and-YR in samples collected from four locations in Hartbeespoort Dam in North West Province (South Africa) during the 2010/2011 summer season. International journal of environmental research and public health, 9(10), pp.3484-3505. |
|
| dc.identifier |
http://hdl.handle.net/20.500.11810/2476 |
|
| dc.identifier |
10.3390/ijerph9103484 |
|
| dc.identifier.uri |
http://hdl.handle.net/20.500.11810/2476 |
|
| dc.description |
Abstract: The Hartbeespoort (HBP) Dam is a reservoir used for agricultural, domestic supply of raw potable water and recreational activities in South Africa’s North-West Province. Eutrophication and cyanobacterial blooms have long been a cause of water-quality problems in this reservoir. The most prevalent bloom-forming species is Microcystis aeruginosa, often producing the toxin microcystin, a hepatotoxin which can negatively impact aquatic animal and human health, and poses a problem for potable water supply. Algal samples were collected monthly from four pre-determined sites in the dam during the summer months (December 2010–March 2011). Intracellular microcystins (MCs) were extracted using SPE C18 cartridges, followed by separation, identification and quantification using LC-ESI-MS techniques. Quantitative variation studies of MCs were conducted with respect to MC congener isolated, sampling site and month. Three main MC congeners (MC-RR, -LR and-YR) were isolated, identified and quantified. In addition, three minor MCs (MC-WR, MC-(H4)YR and (D-Asp3, Dha7)MC-RR were also identified, but were not quantified. The MC dominance followed the order MC-RR>MC-LR>MC-YR across all sites and time. The maximum and minimum concentrations were 268 µg/g and 0.14 µg/g DW for MC-RR and MC-YR, respectively, of the total MCs quantified from this study. One-way ANOVA showed that there were no significant differences between average MC concentrations recorded across months (P = 0.62), there was, however, a marginally-significant difference in concentrations among MC congeners (P = 0.06). ANCOVA revealed a highly significant interaction between sites and MC congeners on MC concentration (P < 0.001). |
|
| dc.language |
en |
|
| dc.subject |
Summer |
|
| dc.subject |
Hartbeespoort Dam |
|
| dc.subject |
Dry weight (DW) |
|
| dc.subject |
Quantitative LC-ESI-MS |
|
| dc.subject |
Intracellular variations |
|
| dc.subject |
Microcystin-RR |
|
| dc.title |
Quantitative Variations of Intracellular Microcystin-LR, -RR and -YR in Samples Collected from Four Locations in Hartbeespoort Dam in North West Province (South Africa) During the 2010/2011 Summer Season |
|
| dc.type |
Journal Article, Peer Reviewed |
|