Assessment of hydropower potential is necessary in evaluating energy generation based on hydrological data for hydropower systems. A study was done at Magunguli waterfalls along the un-gauged Myombwe River with an aim of assessing and analyzing hydropower potential. Hydrological data collections and analysis covered flow rate for gauged Ruaha River and area ratio method to obtain the flow rate for Myombwe River based on catchment area analysed by ArcView GIS. Based on the available resources and data analysis; a hydraulic head of 636 m was realized through GPS techniques, flow rate of 1.979 m3 /s was estimated using area ratio method and flow duration curve. The selected turbine was Pelton turbine which has an efficiency range of 80 - 90%. Based on the hydrological data a Power potential of 9,388.69 kW was realized which is viable for development of mini hydro scheme. To enhance and sustain development of hydropower projects in Tanzania; extensive utilization of acquired data, extended assessment of un-gauge rivers in parallel with impact assessment for the potential hydropower schemes should be done.