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Continuous-flow complete-mixing system for assessing the effects of environmental factors on colony-level coral metabolism

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dc.creator Fujimura, Hiroyuki
dc.creator Higuchi, Tomihiko
dc.creator Shiroma, Kazuyo
dc.creator Arakaki, T.
dc.creator Hamdun, Asha
dc.creator Nakano, Yoshikatsu
dc.creator Oomori, Tamotsu
dc.date 2016-07-14T20:52:55Z
dc.date 2016-07-14T20:52:55Z
dc.date 2008-04
dc.date.accessioned 2018-03-27T08:23:58Z
dc.date.available 2018-03-27T08:23:58Z
dc.identifier Fujimura, H., Higuchi, T., Shiroma, K., Arakaki, T., Hamdun, A.M., Nakano, Y. and Oomori, T., 2008. Continuous-flow complete-mixing system for assessing the effects of environmental factors on colony-level coral metabolism. Journal of biochemical and biophysical methods, 70(6), pp.865-872.
dc.identifier http://hdl.handle.net/20.500.11810/3211
dc.identifier 10.1016/j.jprot.2008.01.014 · Source: PubMed
dc.identifier.uri http://hdl.handle.net/20.500.11810/3211
dc.description A small-scale chamber experimental system was designed to study the effects of temperature on colony-level coral metabolism. The system continuously supplies fresh seawater to the chamber, where it is mixed immediately and completely with the seawater already present. This continuous-flow complete-mixing system (CFCM system), in conjunction with theoretical equations, allows quantitative determination of chemical uptake and release rates by coral under controlled environmental conditions. We used the massive hermatypic coral Goniastrea aspera to examine variations in pH, total alkalinity, and total inorganic carbon for 16 days at 27 degrees C under controlled light intensities (300 and 0 micromol m(-2) s(-1)). We confirmed the stability of the CFCM system with respect to coral photosynthetic and calcification fluxes. In addition, we obtained daily photosynthetic and calcification rates at different temperatures (27 degrees C, 29 degrees C, 31 degrees C, and 33 degrees C). When seawater temperature was raised from 31 degrees C to 33 degrees C, the gross primary production rate (Pgross) decreased 29.5%, and the calcification rate (G) decreased 85.7% within 2 days. The CFCM system allows quantitative evaluation of coral colony chemical release and uptake rates, and metabolism.
dc.language en
dc.subject Calcification
dc.subject Chamber study
dc.subject Continuous-flow complete-mixing model
dc.subject Coral bleaching
dc.subject Metabolism
dc.subject Photosynthesis
dc.subject Temperature effect
dc.title Continuous-flow complete-mixing system for assessing the effects of environmental factors on colony-level coral metabolism
dc.type Journal Article, Peer Reviewed


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