Dissertation ( MSc. Nursing Education)Background: Nursing students faces number of challenges which are associated with interest conflict toward the profession, learning motivation and perceived stress during clinical practice Objectives: To assess the levels of interest, motivation and perceived stress during clinical practice among nursing students in Tanzania Methods: A cross-sectional analytical study design was used to assess levels of interest, motivation and perceived stress during clinical practice among nursing students. 1090 certificate, diploma and undergraduate nursing students attending clinical practice from selected nursing Schools and Universities were selected using convenient and simple random sampling. Results: About 49% of the respondents found to be interested to the nursing profession with 49.7% levels of clinical learning motivation, while the prevalence of perceived stress was 44.3%. Multivariate logistic regression has shown that, students from private institutions (AOR=0.542, p=0.0010) had significantly low stress. Female students were significantly affected by stress as compared to the male counterparts (AOR=1.335, p=0.0259). Undergraduate (AOR=1.940, p=0.0006) students were significantly associated with stress. Students who were not motivated to clinical practice had about 2 times higher odds of being stressed (AOR=1.785, p<0.0001). Conclusion and recommendation: This study has shown a relatively low level of interest to nursing profession and clinical learning motivation among student nurses with a low prevalence of stress during clinical practice.