Browsing by Author "Matee, M. I."

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Detection of Yersinia pestis DNA in human bubo aspirates in Tanzania
(Academic Journals) Ziwa, M. H.; Hang’ombe, B. M.; Lyamuya, E. F.; Kilonzo, B. S; Simulundu, E; Matee, M. I.
Detection of Yersinia pestis DNA in human bubo aspirates in Tanzania
(Academic Journals, 2013-12) Ziwa, M. H.; Hang’ombe, B. M.; Lyamuya, E. F.; Kilonzo, B. S; Simulundu, E; Matee, M. I.
The use of molecular techniques to detect Yersinia pestis has enabled remarkable progress in the provision of necessary information on the occurrence of plague. In Tanzania, despite the long history of plague, DNA confirmation on the presence of Y. pestis in human specimens has not been done. This study was conducted in Mbulu district in Northern Tanzania where plague outbreaks have recently been reported. Nine human bubo specimens were investigated for Y. pestis plasminogen activator gene by using polymerase chain reaction (PCR), and two were found to be positive. The two positive amplicons, together with three previously obtained PCR positive rodent samples, were sequenced using a 3130 genetic analyzer and then compared with those available in GenBank by basic local alignment search tool (BLAST). All sequences obtained from both human and rodent samples showed 99% sequence similarity to Y. pestis plasmid pPCP1, detected from ancient DNA, confirming the presence of Y. pestis in humans that possibly sourced from rodents in Tanzania.
Isolation and potential for transmission of mycobacterium bovis at human–livestock–wildlife interface of the Serengeti Ecosystem, Northern Tanzania
(PubMed) Katale, B. Z.; Mbugi, E. V.; Siame, K. K.; Keyyu, J. D.; Kendall, S.; Kazwala, R. R.; Dockrell, H. M.; Fyumagwa, R. D.; Michel, A. L.; Rweyemamu, M; Streicher, E. M.; Warren, R. M.; Helden, P.; Matee, M. I.
Isolation and potential for transmission of mycobacterium bovis at human–livestock–wildlife interface of the Serengeti Ecosystem, Northern Tanzania
(PubMed, 2015) Katale, B. Z.; Mbugi, E. V.; Siame, K. K.; Keyyu, J. D.; Kendall, S.; Kazwala, R. R.; Dockrell, H. M.; Fyumagwa, R. D.; Michel, A. L.; Rweyemamu, M; Streicher, E. M.; Warren, R. M.; Helden, P.; Matee, M. I.
Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), is a multihost pathogen of public health and veterinary importance. We characterized the M. bovis isolated at the human– livestock–wildlife interface of the Serengeti ecosystem to determine the epidemiology and risk of crossspecies transmission between interacting hosts species. DNA was extracted from mycobacterial cultures obtained from sputum samples of 472 tuberculosis (TB) suspected patients and tissue samples from 606 livestock and wild animal species. M. bovis isolates were characterized using spoligotyping and Mycobacterial Interspersed Repetitive Units-Variable Tandem Repeats (MIRU-VNTR) on 24 loci. Only 5 M. bovis were isolated from the cultured samples. Spoligotyping results revealed that three M. bovis isolates from two buffaloes (Syncerus caffer) and 1 African civet (Civettictis civetta) belonged to SB0133 spoligotype. The two novel strains (AR1 and AR2) assigned as spoligotype SB2290 and SB2289, respectively, were identified from indigenous cattle (Bos indicus). No M. bovis was detected from patients with clinical signs consistent with TB. Of the 606 animal tissue specimens and sputa of 472 TBsuspected patients 43 (7.09%) and 12 (2.9%), respectively, yielded non-tuberculous mycobacteria (NTM), of which 20 isolates were M. intracellulare. No M. avium was identified. M. bovis isolates from wildlife had 45.2% and 96.8% spoligotype pattern agreement with AR1 and AR2 strains, respectively. This finding indicates that bTB infections in wild animals and cattle were epidemiologically related. Of the 24 MIRU-VNTR loci, QUB 11b showed the highest discrimination among the M. bovis strains. The novel strains obtained in this study have not been previously reported in the area, but no clear evidence for recent cross-species transmission of M. bovis was found between human, livestock and wild animals.
Species diversity of non-tuberculous mycobacteria isolated from humans, livestock and wildlife in the Serengeti ecosystem, Tanzania
(BMC Infectious Diseases) Katale, B. Z.; Mbugi, E. V .; Botha, L.; Keyyu, J. D.; Kendall, S.; Dockrell, H. M.; Michel, A. L.; Kazwala, R. R.; Rweyemamu, M. M .; Helden, P. v.; Matee, M. I.