Challenges in determining the pathogenicity status of Leptospira isolates with phenotypic methods: The need for a polyvalent approach

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dc.creatorMgode, G. F.
dc.creatorMachang’u, R. S.
dc.creatorCollares-Pereira, M.
dc.creatorVieira, M. L.
dc.creatorGoris, M. G. A.
dc.creatorEngelbert, M.
dc.creatorHartskeerl, R. A.
dc.date.accessioned2017-04-19T07:10:23Z
dc.date.accessioned2025-08-05T07:17:11Z
dc.date.available2017-04-19T07:10:23Z
dc.date.created2017-04-19T07:10:23Z
dc.date.issued2010-12-04
dc.description.abstractUnderstanding the pathogenic status of leptospires, the causative agents of leptospirosis, is important for successful laboratory diagnosis and control programmes of this zoonosis. Leptospires are difficult to differentiate morphologically; therefore, their pathogenic, intermediate or saprophytic status is currently determined based on both phenotypic tests like growth response in medium containing 8-azaguanine and growth at low temperature (13°C), and genotypic methods. The present study reports on the pathogenic versus saprophytic characterization of nine Leptospira isolates from animal hosts (rodents and dogs) and humans, with specific interest on a canine isolate coded “Dog109”, which showed an ambiguous or intermediate status according to conventional (phenotypic) and molecular (genotypic) tests. The results strongly indicate the need of a polyvalent analytical approach for improving the differentiation of the pathogenic status of circulating serovars,
dc.identifier1996-0808
dc.identifierhttps://www.suaire.sua.ac.tz/handle/123456789/1443
dc.identifier.urihttp://repository.costech.or.tz/handle/20.500.14732/96694
dc.languageen
dc.subjectCharacterization
dc.subjectIsolates
dc.subjectLeptospira
dc.subjectSaprophytic
dc.titleChallenges in determining the pathogenicity status of Leptospira isolates with phenotypic methods: The need for a polyvalent approach
dc.typeArticle

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