Antimicrobial and cytotoxicity Activity of Clausena anisata, Acokanthera shemperii and Olea europaea growing in Tanzania
No Thumbnail Available
Date
Journal Title
Journal ISSN
Volume Title
Publisher
European Journal of Medicinal Plants
Abstract
Description
Aims: To evaluate antimicrobial and cytotoxicity activities of Clausena anisata, Acokanthera
shemperii and Olea europaea against seven Gram negative bacteria and fungal species.
Study Design: Bioassay of antimicrobial assay was done using 96-well micro-dilution method.
Place and Duration of Study: School of Life Science and Bioengineering, Nelson Mandela African
Institution of Science and Technology, Arusha, Tanzania, from April 2014 to June 2014.
Methodology: 96-well micro dilution method was used in antimicrobial assay. Extracts were
loaded in the wells of the first row, followed by serial dilution and 50 μl of the bacterial suspensions
(0.5 MacFarland standard turbidity) were added in each well. The first concentration which showed
no bacterial growth was considered as minimum inhibition concentration. Method developed by Meyer et al 1982 was adopted in cytotoxicity activities.
Results: All extracts indicated antibacterial activity on at least three to five of the tested seven bacteria and two fungi species with MIC value ranging 0.7812 - 12.5 mg/mL. The highest activity
Keywords
Medicinal products from plants