Inhibition of endosome-lysosome system acidification enhances porcine circovirus 2 infection of porcine epithelial cells
| dc.creator | Misinzo, Gerald | |
| dc.creator | Delputte, Peter L | |
| dc.creator | Nauwynck, Hans J | |
| dc.date | 2022-05-19T13:59:32Z | |
| dc.date | 2022-05-19T13:59:32Z | |
| dc.date | 2008-02 | |
| dc.date.accessioned | 2022-10-25T08:52:51Z | |
| dc.date.available | 2022-10-25T08:52:51Z | |
| dc.description | Recently, Misinzo et al. (G. Misinzo, P. Meerts, M. Bublot, J. Mast, H. M. Weingartl, and H. J. Nauwynck, J. Gen. Virol. 86:2057-2068, 2005) reported that inhibiting endosome-lysosome system acidification reduced porcine circovirus 2 (PCV2) infection of monocytic 3D4/31 cells. The present study examined the effect of inhibiting endosome-lysosome system acidification in epithelial cells, since epithelial cells support PCV2 infection in vivo and are used in culturing PCV2 in vitro. Ammonium chloride (NH4Cl), chloroquine diphosphate (CQ), and monensin were used to inhibit endosome-lysosome system acidification. NH4Cl, CQ, or monensin increased PCV2 (Stoon-1010) infection by 726% ± 110%, 1,212% ± 34%, and 1,100% ± 179%, respectively, in porcine kidney (PK-15) cells; by 128% ± 7%, 158% ± 3%, and 142% ± 11% in swine kidney cells; by 160% ± 28%, 446% ± 50%, and 162% ± 56% in swine testicle (S | |
| dc.format | application/pdf | |
| dc.identifier | https://www.suaire.sua.ac.tz/handle/123456789/4192 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/93086 | |
| dc.language | en | |
| dc.publisher | American Society for Microbiology | |
| dc.subject | Endosome-lysosome system acidification | |
| dc.subject | Porcine epithelial cells | |
| dc.title | Inhibition of endosome-lysosome system acidification enhances porcine circovirus 2 infection of porcine epithelial cells | |
| dc.type | Article |