Detection of proteolysis in milk by pseudomonas fluorescens using urea PAGE method

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dc.creatorChove, Lucy Mlipano
dc.creatorAlistair, Grandison
dc.creatorMichael, Lewis
dc.date.accessioned2023-03-06T11:07:50Z
dc.date.accessioned2025-08-05T07:20:12Z
dc.date.available2023-03-06T11:07:50Z
dc.date.created2023-03-06T11:07:50Z
dc.date.issued2017-12-28
dc.description.abstractProteolysis of milk during storage by two strains of Pseudomonas NCIMB 702085 (414) and NCIMB 701274 (416) was investigated using the Urea PAGE method. Pseudomonas fluorescens enzymes were also extracted and purified by dialysis before inoculation into UHT skim milk in an attempt to partially purify the enzyme. Results showed that dialysis removed some peptides and amino acids which would interfere with the assay procedure. The method also confirmed that Pseudomonas fluorescens NCIMB 701274 (416) was more proteolytic than Pseudomonas NCIMB 702085 (414). Thus, Urea PAGE is a useful method for monitoring proteolysis in milk by Pseudomonas fluorescens.
dc.identifier2166-1073
dc.identifierhttp://www.suaire.sua.ac.tz/handle/123456789/4984
dc.identifier.urihttp://repository.costech.or.tz/handle/20.500.14732/97030
dc.languageen
dc.publisherMacrothink Institute
dc.subjectProteolysis
dc.subjectPseudomonas fluorescens
dc.subjectDialysis
dc.subjectUrea PAGE
dc.subjectUHT milk
dc.titleDetection of proteolysis in milk by pseudomonas fluorescens using urea PAGE method
dc.typeArticle

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