A Hydrogenosome with Pyruvate Formate-Lyase: Anaerobic Chytrid Fungi Use an Alternative Route for Pyruvate Catabolism. Mol Microbiol 32:1103-1114

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dc.creatorAkhmanova, Anna
dc.creatorVoncken, Frank
dc.creatorHosea, Ken M.
dc.creatorHarhangi, H.
dc.creatorKeltjens, J. T.
dc.creatorOp Den Camp, H. J.
dc.creatorVogels, G. D.
dc.creatorHackstein, Johannes H. P.
dc.date2016-06-26T17:10:10Z
dc.date2016-06-26T17:10:10Z
dc.date1999
dc.date.accessioned2018-03-27T09:00:38Z
dc.date.available2018-03-27T09:00:38Z
dc.descriptionThe chytrid fungi Piromyces sp. E2 and Neocallimastix sp. L2 are obligatory amitochondriate anaerobes that possesshydrogenosomes.Hydrogenosomes arehighly specialized organelles engaged in anaerobic carbon metabolism; they generate molecular hydrogen and ATP. Here, we show for the ®rst time that chytrid hydrogenosomes use pyruvate formate-lyase (PFL) and not pyruvate:ferredoxin oxidoreductase (PFO) for pyruvate catabolism, unlike all other hydrogenosomes studied to date. Chytrid PFLs are encoded by a multigene family and are abundantly expressed in Piromyces sp. E2 and Neocallimastix sp. L2. Western blotting after cellular fractionation, proteinase K protection assays and determinations of enzyme activities reveal that PFL is present in the hydrogenosomes of Piromyces sp. E2. The main route of the hydrogenosomal carbon metabolism involves PFL; the formation of equimolar amounts of formate and acetate by isolated hydrogenosomes excludes a signi®cant contribution by PFO. Our data support the assumption that chytrid hydrogenosomes are unique and argue for a polyphyletic origin of these organelles.
dc.identifierAkhmanova, A., Voncken, F.G., Hosea, K.M., Harhangi, H., Keltjens, J.T., Op Den Camp, H.J., Vogels, G.D. and Hackstein, J.H., 1999. A hydrogenosome with pyruvate formate‐lyase: Anaerobic chytrid fungi use an alternative route for pyruvate catabolism. Molecular microbiology, 32(5), pp.1103-1114.
dc.identifierhttp://hdl.handle.net/20.500.11810/2687
dc.identifier10.1046/j.1365-2958.1999.01434.x
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2687
dc.languageen
dc.publisherWiley
dc.titleA Hydrogenosome with Pyruvate Formate-Lyase: Anaerobic Chytrid Fungi Use an Alternative Route for Pyruvate Catabolism. Mol Microbiol 32:1103-1114
dc.typeJournal Article, Peer Reviewed

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