Mycobacterium genotypes in pulmonary tuberculosis infections and their detection by trained African giant pouched rats

Mgode, G. F.; Cohen-Bacrie, S.; Bedotto, M.; Weetjens, B. J.; Cox, C.; Jubitana, M.; Kuipers, D.; Machang’u, R. S.; Kazwala, R.; Mfinanga, S.G.; Kaufmann, S. H. E.; Drancourt, M.

dc.creator	Mgode, G. F.
dc.creator	Cohen-Bacrie, S.
dc.creator	Bedotto, M.
dc.creator	Weetjens, B. J.
dc.creator	Cox, C.
dc.creator	Jubitana, M.
dc.creator	Kuipers, D.
dc.creator	Machang’u, R. S.
dc.creator	Kazwala, R.
dc.creator	Mfinanga, S.G.
dc.creator	Kaufmann, S. H. E.
dc.creator	Drancourt, M.
dc.date	2020-06-05T08:41:20Z
dc.date	2020-06-05T08:41:20Z
dc.date	2015
dc.date.accessioned	2022-10-25T08:50:39Z
dc.date.available	2022-10-25T08:50:39Z
dc.identifier	https://www.suaire.sua.ac.tz/handle/123456789/3077
dc.identifier.uri	http://hdl.handle.net/123456789/90474
dc.description	Article of Curr Microbiol (2015) 70:212–218
dc.description	Tuberculosis (TB) diagnosis in low-income countries is mainly done by microscopy. Hence, little is known about the diversity of Mycobacterium spp. in TB infections. Different genotypes or lineages of Mycobacte- rium tuberculosis vary in virulence and induce different inflammatory and immune responses. Trained Cricetomys rats show a potential for rapid diagnosis of TB. They detect over 28 % of smear-negative, culture-positive TB. How- ever, it is unknown whether these rats can equally detect sputa from patients infected with different genotypes of M. tuberculosis. A 4-month prospective study on diversity of Mycobacterium spp. was conducted in Dar es Salaam, Tanzania. 252 sputa from 161 subjects were cultured on Lowenstein-Jensen medium and thereafter tested by rats. Mycobacterial isolates were subjected to molecular iden- tification and multispacer sequence typing (MST) to determine species and genotypes. A total of 34 Mycobac- terium spp. isolates consisting of 32 M. tuberculosis, 1 M. avium subsp. hominissuis and 1 M. intracellulare were obtained. MST analyses of 26 M. tuberculosis isolates yielded 10 distinct MST genotypes, including 3 new genotypes with two clusters of related patterns not grouped by geographic areas. Genotype MST-67, shared by one- third of M. tuberculosis isolates, was associated with the Mwananyamala clinic. This study shows that diverse M. tuberculosis genotypes (n = 10) occur in Dar es Salaam and trained rats detect 80 % of the genotypes. Sputa with two M. tuberculosis genotypes (20 %), M. avium homin- issuis and M. intracellulare were not detected. Therefore, rats detect sputa with different M. tuberculosis genotypes and can be used to detect TB in resource-poor countries.
dc.format	application/pdf
dc.language	en
dc.publisher	Springer Science+Business
dc.subject	Mycobacterium genotypes
dc.subject	Pulmonary tuberculosis infections
dc.subject	Trained African Giant
dc.subject	Pouched Rats
dc.title	Mycobacterium genotypes in pulmonary tuberculosis infections and their detection by trained African giant pouched rats
dc.type	Article