| dc.creator |
Liu, Caizhi Z. |
|
| dc.creator |
He, An Y. |
|
| dc.creator |
Chen, Li-Qiao |
|
| dc.creator |
Samwel Mchele Limbu |
|
| dc.creator |
Wang, Ya W |
|
| dc.creator |
Zhang, Mei L. |
|
| dc.creator |
Du, Zhen-Yu |
|
| dc.date |
2016-06-26T17:11:03Z |
|
| dc.date |
2016-06-26T17:11:03Z |
|
| dc.date |
2016 |
|
| dc.identifier |
Liu, C.Z., He, A.Y., Chen, L.Q., Limbu, S.M., Wang, Y.W., Zhang, M.L. and Du, Z.Y., 2016. Molecular characterization and immune response to lipopolysaccharide (LPS) of the suppressor of cytokine signaling (SOCS)-1, 2 and 3 genes in Nile tilapia (Oreochromis niloticus). Fish & shellfish immunology, 50, pp.160-167. |
|
| dc.identifier |
http://hdl.handle.net/20.500.11810/2690 |
|
| dc.identifier |
10.1016/j.fsi.2016.01.027 |
|
| dc.description |
Suppressor of cytokine signaling (SOCS) proteins are inverse feedback regulators of cytokine and hormone signaling mediated by the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway that are involved in immunity, growth and development of organisms. In the present study, three SOCS genes, SOCS-1, SOCS-2 and SOCS-3, were identified in an economically important fish, Nile tilapia (Oreochromis niloticus) referred to as NtSOCS-1, NtSOCS-2 and NtSOCS-3. Multiple alignments showed that, the three SOCS molecules share highly conserved functional domains, including the SRC homology 2 (SH2) domain, the extended SH2 subdomain (ESS) and the SOCS box with others vertebrate counterparts. Phylogenetic analysis indicated that NtSOCS-1, 2 and 3 belong to the SOCS type II subfamily. Whereas NtSOCS-1 and 3 showed close evolutionary relationship with Perciformes, NtSOCS-2 was more related to Salmoniformes. Tissue specific expression results showed that, NtSOCS-1, 2 and 3 were constitutively expressed in all nine tissues examined. NtSOCS-1 and 3 were highly expressed in immune-related tissues, such as gills, foregut and head kidney. However, NtSOCS-2 was superlatively expressed in liver, brain and heart. In vivo, NtSOCS-1 and 3 mRNA levels were up-regulated after lipopolysaccharide (LPS) challenge while NtSOCS-2 was down-regulated. In vitro, LPS stimulation increased NtSOCS-3 mRNA expression, however it inhibited the transcription of NtSOCS-1 and 2. Collectively, our findings suggest that, the NtSOCS-1 and 3 might play significant role(s) in innate immune response, while NtSOCS-2 may be more involved in metabolic regulation. |
|
| dc.language |
en |
|
| dc.publisher |
Elsevier |
|
| dc.subject |
Nile tilapia (Oreochromis niloticus) |
|
| dc.subject |
Suppressor of cytokine signaling |
|
| dc.subject |
LPS |
|
| dc.subject |
Immune regulation |
|
| dc.title |
Molecular Characterization and Immune Response to Lipopolysaccharide (LPS) of the Suppressor of Cytokine Signaling (SOCS)-1, 2 And 3 Genes in Nile Tilapia (Oreochromis Niloticus) |
|
| dc.type |
Journal Article, Peer Reviewed |
|