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Carbohydrate Analysis of Pulps Using Enzymatic Hydrolysis and High Pressure Liquid Chromato- Graphy

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dc.creator Syverud, Kristin
dc.creator Moe, Størker T.
dc.creator Minja, Rwaichi J. A.
dc.date 2016-07-12T08:27:37Z
dc.date 2016-07-12T08:27:37Z
dc.date 1999
dc.date.accessioned 2018-03-27T08:32:43Z
dc.date.available 2018-03-27T08:32:43Z
dc.identifier Syverud, K., Moe, S.T. and Minja, R.J.A., 1999. CARBOHYDRATE ANALYSIS OF PULPS USING ENZYMATIC HYDROLYSIS AND HIGH PRESSURE LIQUID CHROMATO-GRAPHY. In 10th International symposium on wood and pulping chemistry.
dc.identifier http://hdl.handle.net/20.500.11810/3096
dc.identifier.uri http://hdl.handle.net/20.500.11810/3096
dc.description A revised method for analysis of carbohydrates in pulps is presented. The method utilizes commercially available enzyme preparations for enzymatic hydrolysis of the pulp, followed by acid hydrolysis for complete cleavage of oligomers that are still present. Quantification of monosaccharides in the hydrolysate was performed by HPLC using a Pb-sulphonate/polymer column and deionized water as the mobile phase. Integration of the chromatograms was performed using least squares fitting of partially resolved peaks by commercial software. The method requires moderate operator skills and low investment costs for specialized analytical equipment.
dc.language en
dc.title Carbohydrate Analysis of Pulps Using Enzymatic Hydrolysis and High Pressure Liquid Chromato- Graphy
dc.type Journal Article, Peer Reviewed


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