Purification and Characterization of a Laccase from the Basidiomycete Funalia Trogii (Berk.) Isolated in Tanzania
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Description
A lignolytic basidiomycete fungus, Funalia trogii (Berk.), was isolated from decayed wood in coastal
Tanzania and cultivated in submerged culture. Initially screened crude enzyme filtrate showed complete
rhemazol brilliant blue - R (RBBR) decolorization 2,2â-azino-bis (3-ethylbenzthiazoline)-6-sulfonate and
guaiacol oxidation after 7 days of incubation. The fungal filtrate had maximum laccase activity of 593
U/ml after 15 days of incubation. A laccase was purified by anion exchange and size exclusion
chromatography to good purity as judged by sodium dodecyl sulfate-polyacrylamide gel
electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). The isolated main component had a
molecular weight of ca 58 kDa as determined by MS and an isoelectric point (pI) of 3.8. The optimal pH
and temperature range for the purified laccase were 4.0 - 5.0 and 50 - 70 ÂșC, respectively, using 2, 6-
dimethoxyphenol as a substrate.
Keywords
Funalia trogii, Laccase, Anion exchange chromatography, Size exclusion chromatography, Electrophoresis, Isoelectric focusing