Purification and Characterization of a Laccase from the Basidiomycete Funalia Trogii (Berk.) Isolated in Tanzania

Simple item page
dc.creatorPatrick, Ferdinandi
dc.creatorMtui, Godliving
dc.creatorMshandete, Anthony M.
dc.creatorJohansson, Gunnar
dc.creatorKivaisi, Amelia K.
dc.date2016-05-17T13:31:23Z
dc.date2016-05-17T13:31:23Z
dc.date2009
dc.date.accessioned2018-03-27T09:00:30Z
dc.date.available2018-03-27T09:00:30Z
dc.descriptionA lignolytic basidiomycete fungus, Funalia trogii (Berk.), was isolated from decayed wood in coastal Tanzania and cultivated in submerged culture. Initially screened crude enzyme filtrate showed complete rhemazol brilliant blue - R (RBBR) decolorization 2,2’-azino-bis (3-ethylbenzthiazoline)-6-sulfonate and guaiacol oxidation after 7 days of incubation. The fungal filtrate had maximum laccase activity of 593 U/ml after 15 days of incubation. A laccase was purified by anion exchange and size exclusion chromatography to good purity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). The isolated main component had a molecular weight of ca 58 kDa as determined by MS and an isoelectric point (pI) of 3.8. The optimal pH and temperature range for the purified laccase were 4.0 - 5.0 and 50 - 70 ºC, respectively, using 2, 6- dimethoxyphenol as a substrate.
dc.identifierPatrick, F., Mtui, G., Mshandete, A.M., Johansson, G. and Kivaisi, A., 2009. Purification and characterization of a laccase from the basidiomycete Funalia trogii (Berk.) isolated in Tanzania. Afr. J. Biochem. Res, 3(5), pp.250-258.
dc.identifier1996-0778
dc.identifierhttp://hdl.handle.net/20.500.11810/2112
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2112
dc.languageen
dc.subjectFunalia trogii
dc.subjectLaccase
dc.subjectAnion exchange chromatography
dc.subjectSize exclusion chromatography
dc.subjectElectrophoresis
dc.subjectIsoelectric focusing
dc.titlePurification and Characterization of a Laccase from the Basidiomycete Funalia Trogii (Berk.) Isolated in Tanzania
dc.typeJournal Article, Peer Reviewed

Files

Collections

Department of Molecular Biology and Biotechnology