IGF-1 induces SOCS-2 but not SOCS-1 and SOCS-3 transcription in juvenile Nile tilapia (Oreochromis niloticus).
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The Company of Biologists Limited
Abstract
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Insulin-like growth factor-1 (IGF-1) plays a crucial role in regulating growth in vertebrates while suppressors of cytokine signaling (SOCS) act as feedback inhibitors of the GH/IGF-1 axis. Although SOCS-2 binds IGF-1 receptor and inhibits IGF-1-induced STAT3 activation, presently there is no clear evidence whether IGF-1 could induce SOCS genes expression. The current study aimed to determine whether IGF-1 could induce the transcription of SOCS in juvenile Nile tilapia (Oreochromis niloticus). We show that there is a common positive relationship between the mRNA expression of IGF-I and SOCS-2 under different nutritional statuses and stimulants, but not the expression of SOCS-1 and SOCS-3 mRNA. Furthermore, rhIGF-1 treatment and transcriptional activity assay confirmed the hypothesis that IGF-1 could induce SOCS-2 expression, while has no effect or even decreased the expression of SOCS-1 and SOCS-3. Overall, we obtained evidence that the transcription of SOCS-2, but not SOCS-1 or SOCS-3 could be induced by IGF signaling, suggesting the SOCS-2 serves as a feedback suppressor of IGF-1 axis in juvenile Nile tilapia.
The National Basic Research Program of China (973 program 2014CB138603) and National Natural Science Fund (31472290 and 31772859).
The National Basic Research Program of China (973 program 2014CB138603) and National Natural Science Fund (31472290 and 31772859).
Keywords
Nile tilapia (Oreochromis niloticus), Insulin-like growth factor, Suppressor of cytokine signaling, Transcription