Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomallysosomal system acidification
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Archives virology 2008, Vol. 153: 337–342
Treatment of porcine kidney (PK-15) cells with either interferon-gamma (IFN-g) or endosomallysosomal system acidification inhibitors increases replication of porcine circovirus type 2 (PCV2). In the present study, the effect of a combination of these treatments on the number of infected cells and virus yield was tested. The number of PCV2 (Stoon-1010)-infected PK-15 cells increased in cells treated with ammonium chloride (445 39% increase), IFN-g (446 8%), ammonium chlorideþ IFN-g (1721 283%), chloroquine diphosphate (1037 121%), chloroquine diphosphateþIFN-g (2199 255%), monensin (950 178%) and monensinþIFN-g (1948 60%). Combined IFNg and endosomal-lysosomal system acidification inhibitors increased PCV2 yield by up to 50 times compared to untreated PK-15. This augmented virus replication in PK-15 cells may be helpful in the production of PCV2 vaccines.
Treatment of porcine kidney (PK-15) cells with either interferon-gamma (IFN-g) or endosomallysosomal system acidification inhibitors increases replication of porcine circovirus type 2 (PCV2). In the present study, the effect of a combination of these treatments on the number of infected cells and virus yield was tested. The number of PCV2 (Stoon-1010)-infected PK-15 cells increased in cells treated with ammonium chloride (445 39% increase), IFN-g (446 8%), ammonium chlorideþ IFN-g (1721 283%), chloroquine diphosphate (1037 121%), chloroquine diphosphateþIFN-g (2199 255%), monensin (950 178%) and monensinþIFN-g (1948 60%). Combined IFNg and endosomal-lysosomal system acidification inhibitors increased PCV2 yield by up to 50 times compared to untreated PK-15. This augmented virus replication in PK-15 cells may be helpful in the production of PCV2 vaccines.
Keywords
Porcine circovirus-2, PK-15 cells, Endosomallysosomal system, Interferon-gamma (IFN-g)