Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomallysosomal system acidification

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dc.creatorMisinzo, G.
dc.creatorDelputte, P. L.
dc.creatorLefebvre, D. J.
dc.creatorNauwynck, H. J.
dc.date2017-06-23T06:28:22Z
dc.date2017-06-23T06:28:22Z
dc.date2007-12-17
dc.date.accessioned2022-10-25T08:53:06Z
dc.date.available2022-10-25T08:53:06Z
dc.descriptionArchives virology 2008, Vol. 153: 337–342
dc.descriptionTreatment of porcine kidney (PK-15) cells with either interferon-gamma (IFN-g) or endosomallysosomal system acidification inhibitors increases replication of porcine circovirus type 2 (PCV2). In the present study, the effect of a combination of these treatments on the number of infected cells and virus yield was tested. The number of PCV2 (Stoon-1010)-infected PK-15 cells increased in cells treated with ammonium chloride (445 39% increase), IFN-g (446 8%), ammonium chlorideþ IFN-g (1721 283%), chloroquine diphosphate (1037 121%), chloroquine diphosphateþIFN-g (2199 255%), monensin (950 178%) and monensinþIFN-g (1948 60%). Combined IFNg and endosomal-lysosomal system acidification inhibitors increased PCV2 yield by up to 50 times compared to untreated PK-15. This augmented virus replication in PK-15 cells may be helpful in the production of PCV2 vaccines.
dc.formatapplication/pdf
dc.identifierhttps://www.suaire.sua.ac.tz/handle/123456789/1671
dc.identifier.urihttp://hdl.handle.net/123456789/93358
dc.languageen
dc.subjectPorcine circovirus-2
dc.subjectPK-15 cells
dc.subjectEndosomallysosomal system
dc.subjectInterferon-gamma (IFN-g)
dc.titleIncreased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomallysosomal system acidification
dc.typeArticle

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